한국식물병리학회:학술대회논문집 (Proceedings of the Korean Society of Plant Pathology Conference)
- 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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- Pages.116.1-116
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- 2003
Evaluation of genetic affinities among Fusarium oxysporum f. sp. fragariae by RAPD and rDNA RFLP
- Gopal Nagarajan (Department of Applied Biology, College of Agriculture, Chungnam National University) ;
- Nam, Myeong-Hyeon (Strawberry Experiment Station, Chungnam ARES) ;
- Lee, Yun-Soo (Department of Applied Biology, College of Agriculture, Chungnam National University) ;
- Lee, Woo-Chung (Department of Applied Biology, College of Agriculture, Chungnam National University) ;
- Yoo, Sung-Joon (Bioshield Co. Ltd. HTVC KAIST) ;
- Song, Jeong-Young (Bioshield Co. Ltd. HTVC KAIST) ;
- Kim, Hong-Gi (Department of Applied Biology, College of Agriculture, Chungnam National University)
- 발행 : 2003.10.01
초록
Fusarium oxysporum f. sp. fragariae is a fungal pathogen causing wilt disease on strawberry. The RAPD and RFLP of IGS region of rDNA were used to identify genetic affinity among 22 isolates of F. oxysporum f. sp. fagariae obtained from various location of major strawberry cultivating areas in Korea. Approximately 2.6kb DNA fragment was amplified with primer CNS1 and CNL12, and polymorphisms were observed with Avail and HinfI. A dendrogram was constructed using the UPGMA for cluster analysis. Eight distinct groups were clustered based on the banding pattern obtained from RAPD and rDNA RFLP. There was high level genetic variation among Korean isolates of Fusarium of sporum f. sp. fragariae.
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