Effect of culturing media on biocontrol ability and physiological state of Burkholderia gladioli strain B543.

  • Bae, Yeoung-Seuk (Plant Pathology Division, National Institute of Agricultural Science and Technology) ;
  • Park, Kyungseok (Plant Pathology Division, National Institute of Agricultural Science and Technology) ;
  • Kim, Choong-Hoe (Plant Pathology Division, National Institute of Agricultural Science and Technology)
  • Published : 2003.10.01

Abstract

Long-term repeated culturing of biocontrol agents on a certain medium often results in reduced biocontrol efficacy and altered physiology. Effect of culturing media on biocontrol ability and physiological state of Burkholderia gladioli strain B543 was investigated. Over 20 times repeated cultivation of B. giadioli strain B543 on Kings B medium or nutrient agar medium showed improved biological control of cucumber damping-off caused by Pythium ultimum, while one time cultivation on KB or NA did not. The repeated cultivation also induced the physiological changes of the biocontrol agent such as antifungal activity and the production of protease and siderophore. Our result indicates that adaptation to proper culturing medium can alter biocontrol ability and must consider in optimizing the use of biocontrol agents.

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