한국식물병리학회:학술대회논문집 (Proceedings of the Korean Society of Plant Pathology Conference)
- 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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- Pages.85.2-86
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- 2003
Insertional mutagenesis of fusarium graminearum for characterization of genes involved in disease development and mycotoxin production
- Han, Yon-Kyoung (School of Agricultural Biotechnology, Seoul National University) ;
- Lee, Hyo-Jin (School of Agricultural Biotechnology, Seoul National University) ;
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Yun, Sung-Hwan
(Division of Life Sciences, Soonchunhyang University) ;
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Lee, Yin-Won
(School of Agricultural Biotechnology, Seoul National University)
- 발행 : 2003.10.01
초록
Fusarium graminearum is an important pathogen of cereal crops in many areas of the world causing head blight and ear rot of small grains. In addition to serious economic losses, this fungus produces mycotoxins, such as trichothecenes and zearalenone on diseased crops and has been a potential threat to human and animal health. To massively identify pathogenesis-related genes from F. graminearum, two representative strains (SCKO4 from rice and Z03643 from wheat) were mutagenized using restriction enzyme-mediated integration (REMI). In total, 20,DOD REMI transformants have been collected from the two strains. So far, 63 mutants for several traits involved in disease development such as virulence, mycotoxin production, and sporulation have been selected from 3,000 REMI transformants. Now, selected mutants of interest have being genetically analyzed using a newly developed outcross method (See Jungkwan Lee et al poster). In addition, cloning and characterization of genomic DNA regions flanking the insertional site in the genome of the mutants are in progress.
키워드