한국환경독성학회:학술대회논문집 (Proceedings of the Korea Society of Environmental Toocicology Conference)
- 한국환경독성학회 2003년도 춘계학술대회
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- Pages.180-180
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- 2003
Development and Validation of the Custom Human cDNA Microarray (KISTCHIP-400) for Monitoring Expression of Genes involved in Hormone Disruption
- Kim, Youn-Jung (Toxicology Laboratory, Korea Institute of Science & Technology) ;
- Chang, Suk-Tai (Toxicology Laboratory, Korea Institute of Science & Technology) ;
- Yun, Hye-Jung (Toxicology Laboratory, Korea Institute of Science & Technology) ;
- Jeon, Hee-Kyung (Toxicology Laboratory, Korea Institute of Science & Technology) ;
- Ryu, Jae-Chun (Toxicology Laboratory, Korea Institute of Science & Technology)
- 발행 : 2003.05.01
초록
Transcript profiling is a particularly valuable tool in the field of steroid receptor biology, as these receptors are ligand-activated transcription factors and therefore exert their initial effects through altering gene expression in responsive cells. Also, an increased awareness of endocrine disrupting chemicals (EBCs) and their potential to affect wildlife and humans has produced a demand for practical screening methods to identify endocrine activity. Here we developed an in-house cDNA microarray, named KISTCHIP-400, with 401 clones, hormone related genes, factors, and ESTs, based on public database and research papers. Theses clones contained estrogen, androgen, thyroid hormone St receptors, sex hormone signal transduction & regulation, c-fos, c-myc, ps2 gene, metabolism related genes etc. And to validate the KISTCHIP-400, we investigated gene expression profiles with reference hormones, 10
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