Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)

The Korean Society of Animal Reproduction (한국동물번식학회)
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The Effect of Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) on The Expression of IL-1 System mRNA in Mouse Embryos

  • Kim, D. H. (Aniaml Biotechnology Division, National Livestock Research Institute) ;
  • S. S. Ko (Medical Science Institute, Eulji General Hospital) ;
  • Lee, H. C. (Medical Science Institute, Eulji General Hospital) ;
  • Lee, H. H. (Medical Science Institute, Eulji General Hospital) ;
  • Kim, S. S. (Dept. of OB/GYN, Eulji University School of Medicine) ;
  • Lee, H. J. (Dept. of Physiology, Eulji University School of Medicine) ;
  • B. C. Yang (Aniaml Biotechnology Division, National Livestock Research Institute) ;
  • Park, S. B. (Aniaml Biotechnology Division, National Livestock Research Institute) ;
  • W. K. Chang (Aniaml Biotechnology Division, National Livestock Research Institute)
  • Published : 2003.06.01
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Abstract

Granulocyte-macrophage colony stimulating factor (GM-CSF) is synthesized in the female reproductive tract and has been shown to play an important role in human and murine embryo development and implantation. However, the mechanism of GM-CSF on the embryo development is unknown. Recent studies suggested that GM-CSF may be increase the expression of implantation relented genes, such as interleukin-1 (IL-1) system. Our aim of this study was to compare the interleukin-1$\alpha$ (IL-1$\alpha$), interleukin-1$\beta$ (IL-1$\beta$) and interleukin-1 receptor antagonist (IL-lra) mRNA between the GM-CSF supplemented group and control group in mouse embryos. Mouse 2-cell embryos were cultured in P-1 medium supplemented with or without mouse GM-CSF (10 ng/ml). The number of total and apoptotic cell in blastocyst were assessed by TUNEL. And then, the expression of IL-1$\alpha$, IL-1$\beta$ and IL-1ra mRNA in blastocyst were examined by RT-PCR.

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