한국발생생물학회:학술대회논문집 (Proceedings of the Korean Society of Developmental Biology Conference)
- 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
- /
- Pages.124-124
- /
- 2003
The Effect of Oocyte Activation on Development of Porcine Cloned Embryos
- Kim, Y.S. (College of Veterinary Medicine, Gyeongsang National University) ;
- Lee, S. L. (College of Veterinary Medicine, Gyeongsang National University) ;
- Park, G. J. (College of Veterinary Medicine, Gyeongsang National University) ;
- S. Y. Choe (College of Veterinary Medicine, Gyeongsang National University)
- 발행 : 2003.10.01
초록
The successful development of embryos cloned by nuclear transfer (NT)have been dependent on a wide range of known factors including cell cycle of donor and recipient ooplast, oocyte quality, NT procedure and oocyte activation. The present study compared the development of cloned porcine embryos following different activation treatments. Cumulus-oocyte complexes (COCs) were aspirated from 26 mm follicles of slaughterhouse ovaries and cultured for 22 h in NCSU #23 medium supplemented with 10% porcine follicular fluid, 0.57 mM cysteine, 0.5 g/mL LH, 0.5 g/mL FSH and 10 ng/mL EGF. The COCs were further cultured for an additional 22 h in the same medium at