Proceedings of the Korean Society of Developmental Biology Conference (한국발생생물학회:학술대회논문집)
- 2003.10a
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- Pages.123-123
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- 2003
Effect of IVM Medium and Protein Source on In Vitro Maturation of Canine Oocytes
- Lee, Hyo-Sang (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Yin, Xi-Jun (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Lee, Young-Ho (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Chun, Se-Jin (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Suh, Young-Il (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Park, Keum-Ju (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Seo, Jin-Sung (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Jo, Su-Jin (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University) ;
- Kong, Il-Keun (Department of Animal Science and Technology, College of Agriculture and Life Science, Sunchon National University)
- Published : 2003.10.01
Abstract
This study was conducted to determine the ability of nuclear development of canine oocytes depend on the kind of maturation media and addition of serum sources. Ovaries were collected from a bitches at various stages of estrus cycle by an ovariohysterectomy. Oocytes were collected of cumulus oocytes complexes after slicing of ovaries with blade. The maturation medium was containing 0.6 mM/ml cysteine, 0.2 mM pyruvic acid, 20 ng/ml