한국발생생물학회:학술대회논문집 (Proceedings of the Korean Society of Developmental Biology Conference)
- 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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- Pages.114-114
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- 2003
Effects of Epidermal Growth Factor on the Apoptosis and Implantation Related Genes in Bovine Embryos Developing in vitro
- Park, Sae-Young (Maria Infertility Hospital Medical Institute/ Maria biotechnology) ;
- Tae, Jin-Cheol (Maria Infertility Hospital Medical Institute/ Maria biotechnology) ;
- Kim, Eun-Young (Maria Infertility Hospital Medical Institute/ Maria biotechnology) ;
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Park, Se-Pill
(Maria Infertility Hospital Medical Institute/ Maria biotechnology) ;
- Lim, Jin-Ho (Maria Infertility Hospital) ;
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Kim, Nam-Hyung
(Chungbuk National University)
- 발행 : 2003.10.01
초록
Epidermal growth factor (EGF) induces well-documented mitogenic and differentiating effects on murine and bovine preimplantation embryos. However, the effects of EGF on apoptosis and implantation-related gene expression in bovine embryos developing in vitro have not been evaluated. The objective of this study was to determine the effects of exogenous EGF in the presence and absence of BSA on the preimplantation development of bovine embryos. In addition, we measured cell number, apoptosis, and expression of apoptosis and implantation-related genes of the blastocysts that developed in these culture conditions. In vitro produced bovine embryos were randomly cultured in the same medium containing 0 or 10 ng/ml EGF in the presence and absence of 0.8% BSA. More 2-cell embryos developed into blastocysts at day 7 when BSA was present than when BSA was absent. The addition of 10 ng/