Proceedings of the Korean Society of Developmental Biology Conference (한국발생생물학회:학술대회논문집)
- 2003.10a
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- Pages.102-102
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- 2003
Transforming Growth Factor-$\alpha$ Increases the Yield of Functional Dopaminergic Neurons from in vitro Differentiated Human Embryonic Stem Cells Induced by Basic Fibroblast Growth Factor
- Lee, Keum-Sil (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Shin, Hyun-Ah (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Cho, Hwang-Yoon (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Kim, Eun-Young (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Lee, Young-Jae (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Wang, Kyu-Chang (Seoul National University, Dept. of Neurosurgery) ;
- Kim, Yong-Sik (Seoul National University, Dept of Pharmacology) ;
- Lee, Hoon-Taek (Konkuk University) ;
- Chung, Kil-Saeng (Konkuk University)
- Published : 2003.10.01
Abstract
Embryonic stem (ES) cells proliferate extensively in the undifferentiated state and have the potential to differentiate into a variety of cell types in response to various environmental cues. The generation of functional dopaminergic neurons from ES cells is promising for cell replacement therapy to treat Parkinson's disease. We compared the in vitro differentiation potential of pluripotent human embryonic stem (hES, MB03) cells induced with basic fibroblast growth factor (bFGF) or retinoic acid (RA). Both types of treatment resulted in similar neural cell differentiation patterns at the terminal differentiation stage, specifically, 75% neurons and 11% glial cells. Additionally, treatment of hES cells with brain derived neurotrophic factor (BDNF) or transforming growth factor (TGF)-