Proceedings of the Korean Society of Developmental Biology Conference (한국발생생물학회:학술대회논문집)
- 2003.10a
- /
- Pages.73-73
- /
- 2003
Cryopreservation of Filefish (Thamnaconus septentrionalis) Sperm
- K.H. Kang (Division of Aqua Life Science, Yosu National University) ;
- Z.T. Chen (College of Marine Life Sciences, Ocean University of China) ;
- K.H. Kho (Division of Aqua Life Science, Yosu National University) ;
- Z.F. Zhang (College of Marine Life Sciences, Ocean University of China) ;
- Kim, J.M. (Division of Aqua Life Science, Yosu National University) ;
- Kim, Y.H. (Division of Aqua Life Science, Yosu National University)
- Published : 2003.10.01
Abstract
The present study examined the possibility of long term storage, by cryopreservation in liquid nitrogen, of the sperm of Filefish (Thamnaconus septentrionalis), and the changes in motility, survival rate and ultrastructure of the sperm after freezing and thawing. The sperm was collected by stripping and stored on ice until experiments. For selection of the immobilizing solution, diluted artificial seawater (ASW) of 20, 30 and 40% were tested. The sperm motility was significantly inhibited in 30% ASW, and restored entirely after 100% ASW was added again. Two cryoprotectants, dimethyl sulfoxide (