Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference (한국식품저장유통학회:학술대회논문집)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Effect of gamma-irradiation on the Physicochemical Properties of Hemoglobin

  • Lee, Seung-hwan (Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Song, Kyung-Bin (Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University)
  • Published : 2003.10.01
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Abstract

To elucidate the effect of gamma-irradiation on the molecular properties of hemoglobin, the secondary, tertiary structure, and the molecular weight size of the protein were examined after irradiation at 0.5, 1, 5, and 10 kGy. Gamma-irradiation of hemoglobin solutions caused the disruption of the ordered structure of the protein molecules, as well as degradation, cross-linking, and aggregation of the polypeptide chains. A SDS-PAGE study indicated that irradiation caused initial fragmentation of the proteins and subsequent aggregation due to cross-linking of the protein molecules. The effect of irradiation on the protein was more significant at lower protein concentrations. Ascorbic acid decreased the degradation and aggregation of proteins by scavenging oxygen radicals that were produced by irradiation. A circular dichroism study showed that irradiation decreased the helical content of hemoglobin with a concurrent increase of the aperiodic structure content. Fluorescence spectroscopy indicated that irradiation decreased the emission intensity that was excited at 280 nm.

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