한국생물공학회:학술대회논문집
- 2002.04a
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- Pages.558-561
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- 2002
Proteomic identification of the bovine pregnancy associated proteins by peptide mass fingerprinting
- Kang, Sun-Chul (Dept. of Biotechnology, Daegu University) ;
- Kim, Eun-Jung (Dept. of Biotechnology, Daegu University) ;
- Pyo, Jae-Hoon (Dept. of Animal Science and Biotechnology, Gyungbook National University) ;
- Lim, Jin-Kyu (Dept. of Animal Science and Biotechnology, Gyungbook National University)
- Published : 2002.04.12
Abstract
In this study, a method for the localization of pregnancy-specific proteins from cow urine on 2-D gel have been established. The proteins were digested with trypsin in gel and then analyzed with MALDI- TOF or transferred to a membrane and microsequenced. To examine the pregnancy associated protein spot 2 as a diagnosis marker in bovine urine 2-D western blotting was performed. This antibody was reacted specifically in the protein of pregnant cow’s urine. Consequently spot 2 was identified and found to be good candidates for developing cow pregnancy detection assay kit.
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