한국생물공학회:학술대회논문집
- 2002.04a
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- Pages.232-234
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- 2002
Bacillus cereus에 의한 Phospholipase C (PLC) 생산
- Seo, Guk-Hwa ;
- Lee, Jong-Il ;
- Bornscheuer, Uwe T. (University of Greifswald, Derartment of Biochemistry)
- Published : 2002.04.12
Abstract
Bacillus cereus secretes a nonspecific phospholipase C (PLC) that catalyzes the hydrolysis of phospholipids to yield diacylglycerol and a phosphate monoester. This study focuses on the production of PLC by B. cereus and recombinant E. coli with fusion protein gene (plc::gfp). Fermentation processes have been monitored by a 2-dimensional fluorescence sensor.
공업적으로 중요성이 날로 더해가는 phospholipase C (PLC) 를 Bacillus cereus를 이용하여 생산하였다. 또한, plc::gfp fusion protein 을 생산하는 재조합 E. coli 를 제조하고 배양하였으며 특히 형광센서를 이용하여 PLC 의 생산 특성을 모니터링하였다.
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