Pseudomonas putida BCNU171의 유기용매 내성 및 toluene 내성 관련 유전자의 클로닝

Pseudomonns putida BCNU171 had the tolerant ability to several other organic solvents headed by toluene and xylene. Several mutants were made by mating of BCNUl71, pJFF350 to clarify the structure tolerance gene related. From this mutants the 7 of mutants related with toluene sensitive mutants were selected. pBCNUT-2, pBCNUT-4, pBCNUT-9 was transformed, and from this separated plasmid DNA sequences the gene having high homology was searched. In the case of toluene sensitive mutant it was todX gene (pBCNU4) related with cell membrane, ttgE gene (pBCNU2, pBCNU9) and ttgF gene (pBCNU2, pBCNU9).