Proceedings of the Korea Environmental Mutagen Society Conference (한국환경성돌연변이발암원학회:학술대회논문집)
- 2002.05a
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- Pages.36-42
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- 2002
Accelerated DNA Adduct Formation in the Lung of the Nrf2 Knockout Mouse Exposed to Diesel Exhaust
- Aoki, Yasunobu (Environmental Health Sciences Division, National Institute for Environmental Studies) ;
- Sato, Hiromi (Environmental Health Sciences Division, National Institute for Environmental Studies) ;
- Nishimura, Noriko (Environmental Health Sciences Division, National Institute for Environmental Studies) ;
- Takahashi, Satoru (Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba) ;
- Itoh, Ken (Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba) ;
- Yamamoto, Masayuki (Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba)
- Published : 2002.05.24
Abstract
Diesel exhaust (DE) has been recognized as a noxious mutagen and/or carcinogen, because its components can form DNA adducts. Mechanisms governing the susceptibility to DE and the efficiency of such DNA adduct formation require clarification. The transcription factor Nrf2 is essential for inducible and/or constitutive expression of a group of detoxification and antioxidant enzymes, and we hypothesized that the nrf2 gene knockout mouse might serve as an excellent model system for analyzing DE toxicity. To address this hypothesis, lungs from nrf2(-/-) and nrf2(+/-) mice were examined for the production of xenobiotic-DNA adducts after exposure to DE (3
Keywords
- DNA adduct;
- lung;
- Nrf2;
- phase II enzyme;
- knockout mouse;
- diesel exhaust;
- 8-hydroxydeoxyguanosine;
- oxidative stress;
- polyaromatic hydrocarbon;
- bronchial epithelial cells