대한전기학회:학술대회논문집 (Proceedings of the KIEE Conference)
- 대한전기학회 2002년도 추계학술대회 논문집 전기물성,응용부문
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- Pages.51-53
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- 2002
차세대형 바이오칩의 개발 및 비수식화 표적 DNA를 이용한 유전자 검출
Development of New Biochip and Genome Detection Using an Non-labeling Target DNA
- Choi, Yong-Sung (School of Electrical and Engineering, Wonkwang University) ;
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Park, Dae-Hee
(School of Electrical and Engineering, Wonkwang University) ;
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Kwon, Young-Soo
(Division of Electrical, Electronics and Computer Eng., Dong-A University) ;
- Kawai, Tomoji (The Institute of Scientific and Industrial Research, Osaka University)
- 발행 : 2002.11.09
초록
This research aims to develop a multiple channel electrochemical DNA chip using micro-fabrication technology. At first, we fabricated a high integrated type DNA chip array by lithography technology. Several probe DNAs consisting of thiol group at their 5-end were immobilized on the sold electrodes. Then target DNAs were hybridized by an electrical force. Redox peak of cyclic-voltammogram showed a difference between target DNA and mismatched DNA in the anodic peak current. Therefore, it is able to detect a various genes electrochemically after immobilization of a various probe DNA and hybridization of label-free DNA on the electrodes simultaneously. It suggested that this DNA chip could recognize the sequence specific genes.
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