Proceedings of the KIEE Conference (대한전기학회:학술대회논문집)

The Korean Institute of Electrical Engineers (대한전기학회)
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Development of New Biochip and Genome Detection Using an Non-labeling Target DNA

차세대형 바이오칩의 개발 및 비수식화 표적 DNA를 이용한 유전자 검출

  • Choi, Yong-Sung (School of Electrical and Engineering, Wonkwang University) ;
  • Park, Dae-Hee (School of Electrical and Engineering, Wonkwang University) ;
  • Kwon, Young-Soo (Division of Electrical, Electronics and Computer Eng., Dong-A University) ;
  • Kawai, Tomoji (The Institute of Scientific and Industrial Research, Osaka University)
  • 최용성 (원광대학교 전자재료공학과) ;
  • 박대희 (원광대학교 전자재료공학과) ;
  • 권영수 (동아대학교 전기전자공학부) ;
  • 천합지인 (대판대학 산업과학연구소)
  • Published : 2002.11.09
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Abstract

This research aims to develop a multiple channel electrochemical DNA chip using micro-fabrication technology. At first, we fabricated a high integrated type DNA chip array by lithography technology. Several probe DNAs consisting of thiol group at their 5-end were immobilized on the sold electrodes. Then target DNAs were hybridized by an electrical force. Redox peak of cyclic-voltammogram showed a difference between target DNA and mismatched DNA in the anodic peak current. Therefore, it is able to detect a various genes electrochemically after immobilization of a various probe DNA and hybridization of label-free DNA on the electrodes simultaneously. It suggested that this DNA chip could recognize the sequence specific genes.

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