Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2002.10a
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- Pages.427.2-428
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- 2002
Enhanced p53 Gene Transfer to Human Ovarian Cancer Cells using Cationic Nonviral Vector. DOC
- Choi, Eun-Jeong (College of pharmacy, Seoul national University) ;
- Choi, Sung-Hee (College of pharmacy, Seoul national University) ;
- Park, Jeong-Sook (College of pharmacy, Seoul national University) ;
- Ahn, Woong-Shick (College of Medicine, the Catholic University of Kore) ;
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Kim, Chong-Kook
(College of pharmacy, Seoul national University)
- Published : 2002.10.01
Abstract
Previously we formulated new cationic liposomes, DDC, composed of DOTAP. DOPE, and cholesterol (Chol) in 1 : 0.7 : 0.3 molar ratios, and showed that DDC efficiently deliver the plasmid DNA into ovarian cancer cell lines. Here, wild type p53 DNA was transfected into ovarian cancer cells, using the DOC as a nonviral vector and the expression and activity of p53 gene were evaluated in vitro and in vivo. The complexes of plasmid DNA (pp53-EGFP) and DDC were transfected into OVCAR-3 cells. The gene expression was determined by RT -PCR and western blot analysis. (omitted)
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