Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2002.10a
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- Pages.402.1-402.1
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- 2002
Simultaneous determination of sildenafil and its active metabolite in human plasma using LC/MS/MS
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Ji, Hye-Young
(Drug Metabolism ＆ Bioanalysis Lab, College of Pharmacy, Wonkwang University)
;
- Kim, Sook-Jin (Drug Metabolism ＆ Bioanalysis Lab, College of Pharmacy, Wonkwang University) ;
- Lee, Hong-Il (Drug Metabolism ＆ Bioanalysis Lab, College of Pharmacy, Wonkwang University) ;
- Lee, Seung-Seok (Drug Metabolism ＆ Bioanalysis Lab, College of Pharmacy, Wonkwang University) ;
- Lee, Hye-Sook (Drug Metabolism ＆ Bioanalysis Lab, College of Pharmacy, Wonkwang University)
- Published : 2002.10.01
Abstract
The LC/MS/MS method for the simultaneous determination of sildenafil and its active metabolite N-demethylsildenafil in human plama was developed. Sildenafil. its active metabolite and the internal standard. DA-8159 were extracted form human plasma by liquid-liquid partitioning. A reverse-phase HPLC separation was performed on Luna phenylhexyl column with the mixture of acetonitrile-5 mM ammonium formate (55:45. v/v) as mobile phase. (omitted)
Keywords