Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2002.10a
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- Pages.334.1-334.1
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- 2002
Production, purification and characterization of extracellular protease from Streptomyces scabiei subsp. chosunensis M0137
- Han, Ji-Man (Department of Pharmacy, College of Pharmacy, Chosun University) ;
- Yoo, Jin-Cheol (Department of Pharmacy, College of Pharmacy, Chosun University)
- Published : 2002.10.01
Abstract
Streptomyces scabiei subsp. chosunensis M0137. nonadecanoic acid producer. showed the highest protease activity when grown in OSY medium (oatmeal 1.5%, soybean meal 2%, dried yeast 1 %) supplemented with. glycerol (1 %) and CaCO3 (0.1 %). Two forms of protease(SS-1 and SS-2) were fractionated and purified through Ultrogel AcA 54 gel filtration and DEAE-sepharose CL-6B column chromatography. Both proteases were practically stable in the pH range of 6-10. The optimal pH for the activities of both protease 88-1 and 8S-2 were 7.5 and 8.0. respectively. (omitted)
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