Biochemical analysis of a high-molecular-weight protease from Streptomyces tendae JC412

  • Seong, Chi-Nam (Department of Biology, Sunchon National University) ;
  • Nam, Doo-Hyun (Department of Pharmacy, Yeungnam University) ;
  • Kim, Sung-Jun (Department of Genetic Engineering, Chosun University) ;
  • Cho, Sueung-Sik (Department of Pharmacy, College of Pharmacy, Chosun University) ;
  • Han, Ji-Man (Department of Pharmacy, College of Pharmacy, Chosun University) ;
  • Kim, Joon-Ho (Department of Pharmacy, College of Pharmacy, Chosun University) ;
  • Lee, Hyo-Jung (Department of Pharmacy, College of Pharmacy, Chosun University) ;
  • Yoo, Jin-Cheol (Department of Pharmacy, College of Pharmacy, Chosun University)
  • Published : 2002.10.01

Abstract

Streptomyces tendae JC412 secreted two forms of protease(ST -1 and ST -2) when grown in OSY medium (oatmeal 1.5%. soybean meal 2%. dried yeast 1 %) supplemented with glucose(0.5%) and KH2PO4(0.05%). Initial pH of the culture medium was adjusted to 10.0 with NaOH and incubated at $27^{\circ}C$ on a rotary shaking incubator (180rpm). High- molecular-weight protease ST-1 was heat labile. whereas low molecular protease ST-2(22.000 Da) was reported to be heat stable. (omitted)

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