Isolation. production. purification and biochemical properties of thermostable protease produced by actinomycetes CS0707 isolated from Korean soil.

  • Nam, Doo-Hyun (Department of Pharmacy, Yeungnam University) ;
  • Park, Cheol-Hee (Department of Pharmacology and College of Medicine, Chosun University) ;
  • Kee, Keun-Hong (Department of Patholgy, College of Medicine, Chosun University) ;
  • Kim, Si-Wouk (Department of Environmental Engineering, Chosun University) ;
  • Han, Ji-Man (Department of Pharmacy, College of Pharmacy, Chosun University) ;
  • Kim, Joon-Ho (Department of Pharmacy, College of Pharmacy, Chosun University) ;
  • Lee, Hyo-Jung (Department of Pharmacy, College of Pharmacy, Chosun University) ;
  • Yoo, Jin-Cheol (Department of Pharmacy, College of Pharmacy, Chosun University)
  • Published : 2002.10.01

Abstract

Actinomycetes CS0707 has been isolated in soil sample from location in the Jeju province. Korea, and produces thermostable extracellular proteases. Actinomycetes CS0703 showed the highest protease activity at late exponential phase when grown in OSYM medium (oatmeal 2.0%, soybean meal 1 %, dried yeast 1 %, mannitol 1 %) at $48^{\circ}C$. Three forms of protease(Ta-1, TA-2 and Ta-3) were fractionated by Ultrogel AcA 54 column chromatography, and further purified through ammonium sulfate fractionation, ultramembrane filtration, and DEAE-sepharose CL-6B column chromatography. (omitted)

Keywords