대한약학회:학술대회논문집 (Proceedings of the PSK Conference)
- 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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- Pages.320.2-320.2
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- 2002
Sophoricoside analogs inhibit COX isozymes but not iNOS and TNF in LPS-stimulated macrophages Raw264.7
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Kim, Byung-Hak
(College of Pharmacy. Chungbuk National University) ;
- Min, Kyung-Rak (College of Pharmacy. Chungbuk National University) ;
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Kim, Young-Soo
(College of Pharmacy. Chungbuk National University)
- 발행 : 2002.10.01
초록
Macrophages activated by lipopolysaccharide (LPS) are known to induce several proinflammatory proteins including COX-2. iNOS and TNF which produce chemical mediators involved in inflammatory response. Sophoricoside and its analogs (genistin, genistein and orobol) from Sophora japonica (Leguminosae) showed differential inhibitory effects on COX-1 and 2 activities. Sophoricoside and genistin shwoed IC50 values of 4 uM and 6 uM on COX-2 activity and of 1,497 uM and 135 uM on COX-1 activity, respectively. Genistein and orobol showed IC50 values of 3 uM on COX-2 activity and of 28 uM and 18 uM on COX-1 activity. respectively. Therefore. the legume isoflavonoids to be selective COX-2 inhibitors. However. sophoricoside and its analogs did not show inhibitory effects of COX-2, iNos and TNF transcripts. which were identified by the RT-PCR.
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