Environmentally benign. Background-free protein staining in SDS-polyacrylamide gels using an counter ion-dye complex solution.

  • Jin , Li-Tai (College of Pharmacy, Chonnam National University, Kwangju) ;
  • Hwang, Sun-Young (College of Pharmacy, Chonnam National University, Kwangju) ;
  • Yoo, Gyurng-Soo (College of Pharmacy, Chonnam National University, Kwangju) ;
  • Choi, Jung-Kap (College of Pharmacy, Chonnam National University, Kwangju)
  • 발행 : 2002.10.01

초록

Environmentally benign protein staining method in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using both an acidic dye, zincon (ZC) and a basic dye. ethyl violet (EV) is described. It is based on a counter ion-dye staining technique that employs oppositely charged two dyes to form an ion-pair complex. The selective binding of the free dye molecules to proteins in an acidic solution produces bluish violet colored bands. It is a rapid procedure, involving only fixin and staining steps that are completed in 45 min. (omitted)

키워드