대한약학회:학술대회논문집 (Proceedings of the PSK Conference)
- 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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- Pages.313.2-313.2
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- 2002
Environmentally benign. Background-free protein staining in SDS-polyacrylamide gels using an counter ion-dye complex solution.
- Jin , Li-Tai (College of Pharmacy, Chonnam National University, Kwangju) ;
- Hwang, Sun-Young (College of Pharmacy, Chonnam National University, Kwangju) ;
- Yoo, Gyurng-Soo (College of Pharmacy, Chonnam National University, Kwangju) ;
- Choi, Jung-Kap (College of Pharmacy, Chonnam National University, Kwangju)
- 발행 : 2002.10.01
초록
Environmentally benign protein staining method in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using both an acidic dye, zincon (ZC) and a basic dye. ethyl violet (EV) is described. It is based on a counter ion-dye staining technique that employs oppositely charged two dyes to form an ion-pair complex. The selective binding of the free dye molecules to proteins in an acidic solution produces bluish violet colored bands. It is a rapid procedure, involving only fixin and staining steps that are completed in 45 min. (omitted)
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