Proceedings of the Korean Biophysical Society Conference (한국생물물리학회:학술대회논문집)

The Korean Biophysical Society (한국생물물리학회)
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Structural Characteristics of NiSOD from Streptomyces seoulensis

  • Lee, Jin-Won (Laboratory of Biophysics, School of Biological Sciences, Seoul National University) ;
  • Wuerges, Jochen (Structural Biology Laboratory, Sincrotrone Trieste in Area Science Par) ;
  • Carugo, Kristina Djinovic (Structural Biology Laboratory, Sincrotrone Trieste in Area Science Par) ;
  • Kang, Sa-Ouk (Laboratory of Biophysics, School of Biological Sciences, Seoul National University)
  • Published : 2002.06.01
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Abstract

The heterologous expression of sodN gene from Streptomyces seoulensis in Streptomyces lividans together with the gel filtration and sedimentation equilibrim data indicated that the quaternary structure of NiSOD is homohexamer, which is novel among SODs, not the previously reported homotetramer. The EPR spectrum of $^{61}$ Ni (I = 3/2) substituted NiSOD showed a clear resolved hyperfine structure at g=2.016, unambiguously identifying that the EPR signal from NiSOD is due to Ni.(omitted)

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