Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)

The Korean Society of Animal Reproduction (한국동물번식학회)
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Positive Expression of EGFP in Bovine Embryos after ICSI using Spermatozoa Co-cultured with Exogenous DNA

  • Yoon, Hyo-Jin (Animal Resource Research Center, KonKuk University) ;
  • Han, Sang-Mi (Animal Resource Research Center, KonKuk University) ;
  • Lee, Hoon-Taek (Animal Resource Research Center, KonKuk University) ;
  • Chung, Kil-Saeng (Animal Resource Research Center, KonKuk University)
  • Published : 2002.06.01
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Abstract

There are many methods to introduce exogenous DNA into embryo for the purpose of producing transgenic animals. Exogenous gene can be integrated into oocyte as a form of sperm vector. In this study, sperm was used as a vector for transgene that is enhanced green fluorescent protein (EGFP). The objective of this study was to investigate the expression of exogenous gene in bovine embryos after injection of spermatozoa cocultured with EGFP fragment. Spermatozoa were plunged into liquid nitrogen and thawed several times or shaked in 0.2% Triton X-100 to remove sperm membrane which followed by DTT treatment. (omitted)

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