Optimization of Mouse Embryo Transfer and Superovulation of C57BL/6J Inbred Mice Technique

  • Wang, A.G. (College of Vet. Med., Chungnam National University) ;
  • Han, Y.H. (College of Vet. Med., Chungnam National University) ;
  • Kim, S.U. (Korea Research Institute of Bioscience & Biotechnolog) ;
  • Kim, S.K. (College of Vet. Med., Chungnam National University) ;
  • Yu, D.Y. (Korea Research Institute of Bioscience & Biotechnology)
  • Published : 2002.06.01

Abstract

Currently, the technique of pronuclear microinjection is the most successful and most widely-used method for producing transgenic animals. Among this technique, surperovulation and embryo transfer are the crucial steps for obtaining a large number of fertilized eggs and birth as much as potential founders from the transferred embryos. (omitted)

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