Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2002.06a
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- Pages.18-18
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- 2002
In Vitro Neural Cell Differentiation Derived from Human Embryonic Stem Cells: I. Effect of Neurotrophic Factors on Neural Progenitor Cells
- Kim Eun-Yeong ;
- Jo Hyeon-Jeong ;
- Choe Gyeong-Hui ;
- An So-Yeon ;
- Jeong Gil-Saeng ;
- Park Se-Pil ;
- Im Jin-Ho
- Published : 2002.06.01
Abstract
This study was to investigate the effect of neurotrophic factors on neural cell differentiation in vitro derived from human embryonic stem (hES, MB03) cells. For neural progenitor cell formation derived from hES cells, we produced embryoid bodies (EB: for 5 days, without mitogen) from hES cells and then neurospheres (for 7 - 10 days, 20 ng/㎖ of bFGF added N2 medium) from EB. And then finally for the differentiation into mature neuron cells, neural progenitor cells were cultured in ⅰ) N2 medium (without bFGF), ⅱ) N2 supplemented with brain derived neurotrophic factor (BDNF, 5ng/㎖) or ⅲ) N2 supplemented with platelet derived growth factor-bb (PDGF-bb, 20ng/㎖) for 2 weeks. (omitted)
Keywords
- Human embryonic stem cell;
- Neural progeniter cell;
- In vitro differentiation;
- Neurotrophic factor;
- Mature neuron cell