한국동물번식학회:학술대회논문집 (Proceedings of the KSAR Conference)
- 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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- Pages.5-5
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- 2002
Cell- and Stage-Specific Expression of the Murine nm23-M5 Gene during Late Spermatogenesis and Spermiogenesis
- Hwang Gyu-Chan (Dept. of Dairy Science, Division of Applied Life Science, Gyeongsang National University) ;
- Ok Do-Won (Dept. of Dairy Science, Division of Applied Life Science, Gyeongsang National University) ;
- Lee Mi-Suk (Dept. of Dairy Science, Division of Applied Life Science, Gyeongsang National University) ;
- Kim Jin-Hoe (Dept. of Dairy Science, Division of Applied Life Science, Gyeongsang National University)
- 발행 : 2002.06.01
초록
Nucleoside diphosphate kinases (NDPKs) are conserved through evolution and have been shown to be involved in various biological phenomena. By functional screening in yeast, we identified a new member of the NDPK family, ㎚23-M5, which encodes a 211-amino acid protein with 86% identify to the human homolog, ㎚23-H5. Northern blot analysis reveals that ㎚23-M5 encodes two transcripts of 0.8 and 0.7 kb, which are highly and specifically expressed in adult testis. Reverse transcriptase polymerase chain reaction analysis shows that nm23-M5 first appears in pachytene spermatocytes and increase chain reaction in abundance through subsequent stages. (omitted)