Proceedings of the Korea Society of Poultry Science Conference (한국가금학회:학술대회논문집)
- 2002.11a
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- Pages.98-99
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- 2002
The Production of Germline Chimeras by Transfer of Gonadal Primordial Germ Cells Separated with Magnetic Cell Sorter System in Quail
- Shin, Sang-Su (School of Agricultural Biotechnology, Seoul National University) ;
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Park, Tae-Sub
(School of Agricultural Biotechnology, Seoul National University) ;
- Kim, Mi-Ah (School of Agricultural Biotechnology, Seoul National University) ;
- Kim, Jin-Nam (School of Agricultural Biotechnology, Seoul National University) ;
- Lee, Kyung-Joo (School of Agricultural Biotechnology, Seoul National University) ;
- Park, Hyun-Jeong (School of Agricultural Biotechnology, Seoul National University) ;
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Han, Jae-Yong
(School of Agricultural Biotechnology, Seoul National University)
- Published : 2002.11.01
Abstract
Collection of large number of gonadal Primordial germ cells(gPGCs) is a prerequisite factor for improving germline transmission efficacy in the aves, In this study a magnetic-activated cell sorter(MACS) was applied for improving retrieval efficacy of quail gPGCs and the migration capacity of MACS-separated gPGCs was further examined after being transplanted to recipient embryos. We also induced germline chimeras by transfer of MACS-separated quail gPGCs at the efficiency of 17.4% on average.