Proceedings of the Korean Society of Toxicology Conference (한국독성학회:학술대회논문집)
- 2002.05b
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- Pages.59-72
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- 2002
GAP JUNCTION, A BIOMARKER FOR CANCER AND CHEMOPREVENTION: PREVENTIVE EFFECT OF EPICATECHIN AND GINSENOSIDE $Rb_$ ON THE INHIBITION OF GAP JUNCTIONAL INTERCULLULAR COMMUNICATION BY TPA AND $H_2O_2$
- Kang, Kyung-Sun (College of Veterinary Medicine, Seoul National University, Suwon) ;
- Lee, Yong-Soom (College of Veterinary Medicine, Seoul National University, Suwon)
- Published : 2002.05.03
Abstract
The anticarcinogenic effects of epicatechin(EC) and ginsenoside Rb2(Rb2), which are major components of green tea and Korea ginsen, respectively, were investigated using a model system of gap junctional intercellular communication (GJIC) in WB-F344 rat liver epithelial cells. 12-O-Tetradecanoylphorbol-13-accetate (TPA) and hydrogen preoxide, known as cancer promoters, inhibited GJIC in the epithelial cells as determined by the scrape loading/dye transfer assay, fluorescence redistribution assay after photobleaching, and immunofluorescent staining of connexin 43 using a laser confocal microscope. The inhibition of GJIC by TPA and H2O2 was prevented with treatment of Rb2 or Ec. The effect of EC on GJIC was stronger in TPA-treated cells than in H2O2-treated cells, while the effect of Rb2 was opoosite to that of EC. EC, at the concentration of 27.8
Keywords
- Green tea;
- Ginseng;
- Catechins;
- Ginsenosides;
- Gap junctional intercellular communicaion(GJIC);
- Connexin 43