해양생물 유래 Pseudomonas aeruginosa BYK -2(KCTC 18012P)로부터 biosurfactant 생산성 향상을 위한 fed-batch 배양

In order to maximize the cell growth and the biosurfactant production by the Pseudomonas aeruginosa BYK-2(KCTC 18012P), in the fed-batch fermentation processes were performed varying the feeding medium concentrations and the feeding rate. Feel-batch culture was performed with the optimal agitation speed of 200rpm and the aeration rate of 0.67vvm in a 7L Jar fermentor containing 3L of modified medium and 2.0-2.5%(v/v) fish oil as a carbon source. Addition of fish oil(2.5mL/l00mL modified medium), when fish oil was depleted, the cell and biosurfactant concentration were 6.1g/L and 22.7g/L, respectively.