한국미생물생명공학회:학술대회논문집 (Proceedings of the Korean Society for Applied Microbiology Conference)
- 한국미생물생명공학회 2001년도 Proceedings of 2001 International Symposium
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- Pages.149-153
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- 2001
Production of pediocin by Chemical Synthesis and Bactericidal Mode of Action
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Koo, Min-Seon
(Korea Food Research Institute) ;
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Kim, Wang-June
(Korea Food Research Institute) ;
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Kwon, Dea-Young
(Korea Food Research Institute) ;
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Min, Kyung-Hee
(Sookmyung Women's University)
- 발행 : 2001.06.01
초록
To investigate the mode of bactericidal action for antimicrobial peptide, pediocin, synthetic and mutant pediocins were prepared by direct chemical synthesis. Native pediocin was purified from Pedio-coccus acidilactici M and its conformational structure and bactericidal functions were analyzed and compared to synthetic pediocin. Schematic mode of pediocin actions, how pediocin binds on the target cell membrane, penetrates and makes tunnel are proposed. For these purposes, primary and secondary structures of pediocin was analyzed and disulfide bond assignment was also done. The pediocin purified from P. acidilactici M had high effective bactericidal ability against gram positive bacteria, especially Listeria monocytogenes and was very stable at extreme pHs and even at high temperatures such as autoclaving temperature (121
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