Proceedings of the Korean Environmental Sciences Society Conference (한국환경과학회:학술대회논문집)
- 2001.05a
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- Pages.238-239
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- 2001
An Enzyme-Linked Immunosorbent Assay for Carp (Cyprinus carpio) Vitellogenin and Assay for Oestrogenic Chemicals
- Jeong, Jing-Woon (School of Environmental System Inje University) ;
- Park, Eon-Jung (Department of Chemistry, Pusan National University) ;
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Kim, Andre
(Department of Chemistry, Pusan National University) ;
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Park, Jang-Su
(Department of Chemistry, Pusan National University) ;
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Park, Heung-Jai
(School of Environmental System Inje University)
- Published : 2001.05.01
Abstract
The egg-yolk precursor vitellogenin(VTG) is secreted by the liver of female and male fish, in response to estrogenic compound. Carp(Cryprinus carpio) vitellogenin of one major protein is 160kDa, two minor proteins is 110kDa and 170kDa. We were induced vitellogenin by inject of 17-estradiol and purified in a two step procedure by separating it from plasma protein precipitated by 35% saturated