한국생물물리학회:학술대회논문집 (Proceedings of the Korean Biophysical Society Conference)

한국생물물리학회 (The Korean Biophysical Society)
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Nickel Binding Protein (NBPI) and Its Gene from Streptomyces seoulensis

  • Kim, In-Kwon (Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University) ;
  • Sa-Ouk kang (Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University)
  • 발행 : 2001.06.01
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초록

Nickel-binding protein (NBP1) was purified from the crude extract of Streptomyces seoulensis using Ni$^{2＋}$-charged metal chelate affinity chromatography. The molecular mass of NBPI determined on SDS-PAGE was 38kDa. An approximately 3 kb DNA fragment containing the structural gene for NBP1 was cloned from lEMBL3 genomic library of S. seoulensis using a DNA fragment PCR-amplified with the primers designed from N-terminal and internal amino acid sequences of NBP1.(omitted)

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