Proceedings of the Korean Society of Toxicology Conference (한국독성학회:학술대회논문집)
- 2001.10a
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- Pages.188-188
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- 2001
SYNERGISTIC EFFECT OF HUMAN CYTOCHROME B5 COEXPRESSION ON THE METABOLIC ACTIVITY OF CYP1A2 IN CHINESE HAMSTER OVARY CELLS
- Kang, Jin-Sun (College of Medicine and Institute of Biomedical Sciences, Hanyang University) ;
- Kang, Hyuck-Joon (College of Medicine and Institute of Biomedical Sciences, Hanyang University) ;
- Dong, Mi-Sook (Graduate School of Biotechnology, Korea University) ;
- Park, Chang-Hwan (College of Medicine and Institute of Biomedical Sciences, Hanyang University)
- Published : 2001.10.01
Abstract
Human cytochrome B5 (CYB5) was coexpressed with cytochrome P450 1A2 (CYP1A2), NADPH-CYP450 reductase (CYPR) and Ν-acetyltransferase 2 (NAT2) in Chinese hamster ovary (CHO) cells. The expression of four proteins was determined by Western blot analyses. The introduction of cDNAs to CHO cells were transduced via retroviral vectors. The cytotoxicity assay of 2-aminoanthracene (2-AA) and aflatoxin B
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