Proceedings of the Korean Society of Toxicology Conference (한국독성학회:학술대회논문집)

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Evaluation of neutral red uptake assay using Balb/c 3T3 cells as a screening test to predict skin phototoxicity potential

  • Lee, Jong-Kwon (Department of Toxicology, National Institute of Toxicology Research, Korea Food and Drug Administration) ;
  • Lee, Eun-Hee (Department of Toxicology, National Institute of Toxicology Research, Korea Food and Drug Administration) ;
  • Kim, Ju-Hwan (Department of Toxicology, National Institute of Toxicology Research, Korea Food and Drug Administration) ;
  • Hong, Jin-Tae (Department of Toxicology, National Institute of Toxicology Research, Korea Food and Drug Administration) ;
  • Kim, Hyung-Soo (Department of Toxicology, National Institute of Toxicology Research, Korea Food and Drug Administration) ;
  • Park, Ki-Sook (Department of Toxicology, National Institute of Toxicology Research, Korea Food and Drug Administration) ;
  • Ahn, Kwang-Soo (Department of Toxicology, National Institute of Toxicology Research, Korea Food and Drug Administratio) ;
  • Cho, Dae-Hyun (Department of Toxicology, National Institute of Toxicology Research, Korea Food and Drug Administration) ;
  • Lee, Sun-Hee (Department of Toxicology, National Institute of Toxicology Research, Korea Food and Drug Administration)
  • Published : 2001.05.01
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Abstract

In order to evaluate the neutral red uptake assay as an alternative method for phototoxicity test, we compared the potential of phototoxicity in vitro in cultured human fibroblasts and 3T3 fibroblast cells derived from Balb/c mice. Both fibroblasts were exposed to various known phototoxic chemicals (promethazine, neutral red, chlorpromazine, chlortetracycline, amiodarone, bithionol, 8-methoxypsoralen) and non-phototoxic chemical (ammonium laureth sulfate) and irradiated with 5 J/cm$^2$ of UVA.(omitted)

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