An in-vitro investigation of appearance of smear layer removal depending on application times and temperature of EDTA

The objective of this study was to evaluate the effect of EDTA irrigant depending on application times and temperatures. 31 human mature extracted teeth with a single canal and a closed apex were sectioned with microtome in 3mm thickness. They were distributed randomly into 6 groups of 10 specimens each and control group of 2 specimens. Each specimen was prepared with $GT^{TM}$ rotary file (Dentsply, Maillefer Co., Swiss) and irrigated with 3ml sodium hypochlorite every minute. Then smear layer wasn removed with EDTA solution ($PULPDENT^{\circledR}$, PULPDENT Co., USA.) except two control specimens. Specimens of each group were irrigated with 17％ EDTA.(omitted)