한국응용약물학회:학술대회논문집 (Proceedings of the Korean Society of Applied Pharmacology)
- 한국응용약물학회 2001년도 추계학술대회 및 정기총회
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- Pages.114-114
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- 2001
CYP1A1 GENE EXPRESSION IS DOWN REGULATED BY HYPOXIC AGENTS
- Joung, Ki-Eun (College of pharmacy, Ewha Woman′s University) ;
- Kim, Yeo-Woon (College of pharmacy, Ewha Woman′s University) ;
- Syrie Pang (College of pharmacy, Ewha Woman′s University) ;
- Sheen, Yhun-Yhong (College of pharmacy, Ewha Woman′s University)
- 발행 : 2001.11.01
초록
Since hypoxia-inducible factor-lalpha (HIF-lalpha) and the arylhydrocarbon receptor (AhR) shared the AhR nuclear translocator (Arnt) for hypoxia- and AhR-mediated signaling, respectively, it was possible to establish the hypothesis that hypoxia could regulate Cyplal expression. In order to understand the mechanism of Cyplal gene expression, we demonstrated here that hypoxic agents such as cobalt chloride, desferrioxarnine, and picolinic acid reduced the TCDD induced Cyplal promoter activity based on the determination of luciferase activity in Hepa I cells transfected with pmCypla1-Luc. Also cobalt chloride inhibited the TCDD stimulated Cyplal mRNA level as well as EROD activities in both Hepa I and MCF-7 cells. Hypoxic agents such as cobalt chloride, picolinic acid, and desferrioxamine showed inhibition of luciferase activity that was induced by lnM TCDD treatment with dose dependent manner. Concomitant treatment of 150
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