Proceedings of the Korean Society of Near Infrared Spectroscopy Conference (한국근적외분광분석학회:학술대회논문집)
- 2001.06a
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- Pages.3102-3102
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- 2001
ANTIOXIDATIVE ACTIVITIES OF SOME DIETARY FIBERS DETERMINED BY AN NIR EMISSION SPECTROSCOPY
- Suzuki, Nobutaka (Dept. of Food Science, Shimonoseki Univ. of Fisheries) ;
- Nagai, Takeshi (Dept. of Food Science, Shimonoseki Univ. of Fisheries) ;
- Tokunou, Kazunari (Dept. of Food Science, Shimonoseki Univ. of Fisheries) ;
- Mizumoto, Iwao (Toyama National Inst. of Maritime Tech.) ;
- Matsuya, Hiroko (Koriyama Womens Univ.) ;
- Yoda, Binkoh (Koriyama Womens Univ.) ;
- Itami, Toshiaki (Dept. of Aquaculture, Shimonoseki Univ. of Fisheries) ;
- Takahashi, Yukinori (Dept. of Aquaculture, Shimonoseki Univ. of Fisheries) ;
- Kozawa, Akiya (ITE/IBA Research Inst.)
- Published : 2001.06.01
Abstract
Constituents of several .representative seaweeds, such as wakame Undaria pinnatifida; hijikia Hizikia fusifome; and kombu Laminaria japonica, were found to have fairly large reaction rates determined by quenching experiments of emission spectra in the near-infrared region (1max: 1270 nm) from singlet oxygen (102). Emission spectra of singlet oxygen generated from an aqueous solution of Rose Bengal under irradiation with a green laser (330 nm) were measured by a near-infrared (NIR) emission spectrometer constructed in our laboratory. The quenching experiments were as follows: Intensities of emission spectra were measured in the absence (I0) and in the presence of the seaweed constituents (I): Ratios of I0/I were plotted against every concentration of the quenchers (Stern-Volmer plots) which gives a straight line. The slope of each line gives a kqt value which gives a quenching constant kq value (an antioxidative constant against singlet oxygen) when the t value (half-life time of singlet oxygen in the solvent used) was given. The determined reaction rates are between 103-105 (g/l)-ls-1; the larger ones are as large as that of ascorbic acid, 8.4
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