Proceedings of the Korean Society of Near Infrared Spectroscopy Conference (한국근적외분광분석학회:학술대회논문집)
- 2001.06a
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- Pages.1628-1628
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- 2001
Thermal denaturation analysis of protein
- Miyazawa, Mitsuhiro (National Institute of Sericultural and Entomological Science(NISES))
- Published : 2001.06.01
Abstract
Near infrared (NIR) spectroscopy is a powerful technique for non-destructive analysis that can be obtained in a wide range of environments. Recently, NIR measurements have been utilized as probe for quantitative analysis in agricultural, industrial, and medical sciences. In addition, it is also possible to make practical application on NIR for molecular structural analysis. In this work, Fourier transform near infrared (FT-NIR) measurements were carried out to utilize extensively in the relative amounts of different secondary structures were employed, such as Iysozyme, concanavalin A, silk fibroin and so on. Several broad NIR bands due to the protein absorption were observed between 4000 and
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