한국동물번식학회:학술대회논문집 (Proceedings of the KSAR Conference)
- 한국동물번식학회 2001년도 춘계학술발표대회
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- Pages.49-49
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- 2001
Differential Inheritance Modes of DNA Methylation between Euchromatic and Heterochromatic DNA Sequences in Ageing Fetal Bovine Fibroblasts
- Y.K. Kang (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- D.B. Koo (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- Park, J.S. (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- Park, Y.H. (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- Lee, K.K. (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- Y.M. Han (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology)
- 발행 : 2001.03.01
초록
To elucidate overall changes in DNA methylation that occurs by inappropriate epigenetic control during ageing, we compared fetal bovine fibroblasts and their aged neomycin-resistant versions using bisulfite-PCR technology. Reduction in DNA methylation was observed in euchromatic repeats (18S-rRNA/art2) and promoter regions of sing1e-copy genes (the cytokeratin/-lactoglobulin/interleukin-13 genes). Contrastingly, a stable maintenance of DNA methylation was revealed in various heterochromatic sequences (satellite I/IIalphoid and Bov-B). The differential inheritance modes of DNA methylation was confirmed through the analysis of individual neomycin-resistant clones. These global, multi-loci analyses provide evidence on the tendency of differential epigenetic modification between genomic DNA regions during ageing.