한국동물번식학회:학술대회논문집 (Proceedings of the KSAR Conference)
- 한국동물번식학회 2001년도 춘계학술발표대회
- /
- Pages.43-43
- /
- 2001
The Effect of Fertilization-Promoting Peptide on Fertilizing Ability and Glycosidase Activity In Vitro of Frozen-Thawed Boar Spermatozoa
- Hwang, I.S. (College of Animal Resource Science, Kangwon University) ;
- Park, C.K. (College of Animal Resource Science, Kangwon University) ;
- H.T. Cheong (College of Animal Resource Science, Kangwon University) ;
- B.K. Yang (College of Animal Resource Science, Kangwon University) ;
- Kim, C.I. (College of Animal Resource Science, Kangwon University)
- 발행 : 2001.03.01
초록
This study evaluated the effect of fertilization-promoting peptide (FPP) on fertilizing ability and glycosidase activity in vitro of spermatozoa frozen-thawed in pig, Using chlortetracycline fluorescence analysis, the various glycosidase analyses and the oocyte penetration test, we have obtained evidence that FPP can promote the fertilizing ability and glycosidase activity of frozen-thawed spermatozoa in vitro. When frozen-thawed spermatozoa was washed with different concentrations of FPP, there were significantly (P<0.05) more acrosome-reacted in medium with 100 nM than 0, 50, 200 and 400 nM. The penetration rates were also highest in medium containing with 100 nM FPP (P<0.05). On the other hand, the