Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2001.03a
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- Pages.7-7
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- 2001
Efficient Cryopreservation of Hanwoo (Korean Cattle) Blastocysts Derived from Nuclear Transfer with Somatic Cell Using Vitrification
Abstract
Cryopreservation by vitrification of Hanwoo blastocysts derived from nuclear transfer with Hanwoo adult ear cell was compared with that of in vitro fertilized blastocysts. For vitrification, day 7 or day 8 blastocysts were serially exposed in glycerol (G) or/and ethylene glycol (EG) mixtures (10% G for 5 min, 10% G plus 20% EG for 5 min, and 25% G plus 25% EG for 30 sec) which was diluted in 10% FBS added D-PBS. Thawing of straw was carried out in air for 10 sec and then in water bath of