Proceedings of the Korean Society of Developmental Biology Conference (한국발생생물학회:학술대회논문집)
- 2001.10a
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- Pages.18-24
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- 2001
Expression Profiling of Genes involved in the Control of Pluripotency Using CDNA Microarray
- Lee, Young-Jin (Department of Life Science, College of Natural Sciences, Hanyang University) ;
- Hong, Seok-Ho (Department of Obstetrics and Gynecology, College of Medicine) ;
- Nah, Hee-Young (Department of Obstetrics and Gynecology, College of Medicine) ;
- Chae, Jai-Hyung (Department of Life Science, College of Natural Sciences, Hanyang University) ;
- Jung, Ho-Sun (Department of Life Science, College of Natural Sciences, Hanyang University) ;
- Kim, Beom-Sue (Department of Life Science, College of Natural Sciences, Hanyang University) ;
- Kim, Chul-Geun (Department of Life Science, College of Natural Sciences, Hanyang University)
- Published : 2001.10.01
Abstract
To identify genes implicated in the control of pluripotency as well as characteristics of stem cells, we analyzed expression profiles of genes derived from mouse morulas, blastocysts, embryonic stem cells, mesenchymal stem cells, and uterus tissue cDNA microarray. Comparative analyses of their expression profiles identified putative clones that expressed specifically in specific samples or not in a specific sample. The expression pattern of these candidate clones was analyzed using RT-PCR and non-radioactive in situ hybridization. Functional annotation of these clones on pluripotency and stem cells and molecular mechanisms underlying many facets of mammalian development and differentiation.
Keywords