Proceedings of the Microbiological Society of Korea Conference (한국미생물학회:학술대회논문집)

The Microbiological Society of Korea (한국미생물학회)
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In Situ Identification of Cyanobacteria

  • Ahn Tae-Seok (Dept. of Environmental Science, Kangwon National University) ;
  • Hong Sun-Hee (Dept. of Environmental Science, Kangwon National University) ;
  • Chung Hyun-Mi (National Institute of Environmental Research (NIER)) ;
  • Belkova Natalia L. (Limnological Institute SB RAS.)
  • Published : 2001.11.01
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Abstract

Seven cyanobacteria strains (Anabaena macrospora NIERl0016, Oscillatoria sp. NIER10042, Microcystis aeruginosa NIER10015, M. ichtyoblabe BIER10025, BIER10040, M. novacekii NIER10029, M. wesenbergii NIER10068) were tested with four rRNA - targeted oligonucleotide probes labelled with horseradish peroxidase (HRP) and specific for cyanobacteria. Non- fluorescent detection of hybridization signal was used. The hybridization with artificial mixture of cyanobacteria have shown the possibility to use 2 species-specific probes in duplicate hybridization and detection with different colored substrates.

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