한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Human Cardiac Troponin I 면역분석을 위한 단일클론 항체의 특성화

  • 오흥일 (고려대학교 생명공학원, 바이오센서 시스템공학 연구실) ;
  • 양진아 (고려대학교 생명공학원, 바이오센서 시스템공학 연구실) ;
  • 백의환 (고려대학교 생명공학원, 바이오센서 시스템공학 연구실) ;
  • 백세환 (고려대학교 생명공학원, 바이오센서 시스템공학 연구실)
  • Published : 2000.11.09
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Abstract

Six monoclonal antibodies to human cardiac troponin I (hcTnI) were produced to eventually develop an immunosensor for acute myocardial infarction (AMI). For the characterization of these antibodies, a set of 11 different peptides covering selected ranges of the complete amino acid sequence of hcTnI was prepared and used for epitope mapping. Such analysis allowed to select an appropriate pair of antibodies that can form a sandwich type of immune complexes and was consequently used for an immunoassay.

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