한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Pseudomonas aeruginosa BYK-2의 균체고정화법을 이용한 생물유화제의 생산

  • 정혜성 (부경대학교 생물공학과) ;
  • 김학주 (부경대학교 생물공학과) ;
  • 하순득 (부경대학교 생물공학과) ;
  • 황선희 (부경대학교 생물공학과) ;
  • 구헌서 (동명대학 공업화학과) ;
  • 공재열 (부경대학교 생물공학과)
  • Published : 2000.04.08
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Abstract

The optimal conditions and properties for the immobilization of marine bacterium Pseudomonas aeruginosa BYK-2 have been determined. For the high productioon of biosurfactant, Na-alginate, PVA, modified PVA were used as a carrier. The optimal emulsifying activity on immobilized Pseudomonas aeruginosa BYK-2 showed 1036Unit (about 2.2g/L biosurfactant) in Basal salt medium(B.S.M.) at $25^{\circ}C$, 100rpm. Ca-alginate was selected the optimal bead among PVA, modified PVA and Ca-alginate. The optimal cell load in alginate bead was 10 gCWW/100g carrier. As the results of incubation of immobilized 5g Ca-alginate bead (conditions; 3% alginate, bead diameter: 2.3mm, 10% cell load) in 50m1 production medium, The emulsifying activity of 1407Unit, about 3.0g/L biosurfactant was obtained from immobilized cell after cultivation of 92hr at $25^{\circ}C$, 100rpm.

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