한국생물공학회:학술대회논문집
- 2000.04a
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- Pages.215-218
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- 2000
IMPROVEMENT OF GENETIC TRANSFORMATION SYSTEM IN ASPERGILLUS ORYZAE
- Lee, Jae-Won (Department of Biotechnology, Chung-Ang University) ;
- Hahm, Young-Tae (Department of Biotechnology, Chung-Ang University)
- Published : 2000.04.08
Abstract
Aspergillus oryzae is a filamentous fungus classified in the group Aspergillaceae Ascomycetes. It is an important microorganism for industrial production of enzymes and fermented food productions. The genetic transformation system in A. oryzae was used to protoplast mediated transformation with PEG/
A. oryzae에 있어서 protoplast를 이용한 형질전환이 아닌 세포벽이 부분적으로 분해된 cell을 이용하여 electroporation으로 형질전환시켰고, novozyme234, hemicellulase와 celluclast를 사용하여 형질전환 효율이 어떻게 다른지를 비교 분석 하였다. Hemicellulase를
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