한국생물공학회:학술대회논문집
- 2000.04a
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- Pages.57-58
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- 2000
Production of Dye-decolorizing Enzyme using Molasses-containing Medium
- Lee, Tae-Ho (Research Laboratory of Resources utilization, Tokyo Institute of Technology, Institute for Environmental Technology and Industry, Pusan National University) ;
- Shoda, Makoto (Research Laboratory of Resources utilization, Tokyo Institute of Technology)
- Published : 2000.04.08
Abstract
Production of dye-decolorizing enzyme was investigated with the cultivation of Geotrichum candidum Dec1 (abbreviated to Dec1) using molasses as a cheap raw materials. Molasses was found to stimulate the enzyme production as well as a role of carbon source, because the production increased with molasses content up to 50 g/L. However, the severe inhibition of dye-decolorizing enzyme activity was observed even at low concentration of molasses 10 g/L when purified decolorizing peroxidase was used. Its inhibitory effect was reduced through the cultivation of Dec1. The fractions of molasses separated by a gel chromatography showed the different degrees of inhibition. As a way to reduce the inhibitory effect, the dilution of culture broth was examined, and the total decolorizing activity for Reactive blue 5 increased 7 times as much as that of original culture broth by 30 times dilution. On the basis of result, we proposed a process scheme which can fully utilize both positive and negative effect of molasses in dye-decolorizing process using molasses.
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